Policies and Pricing
Next Generation DNA Sequencing (PacBio Revio and Sequel II)
|SMRT Cell type||Internal rate||External rate|
|25M SMRT cell (Revio), 24 hours||$1250||$1850|
|8M SMRT cell (Sequel II), 15 hours||$1490||$2120|
|8M SMRT cell (Sequel II) 30 hours||$1890||$2620|
Five micrograms of DNA should be provided to enable library construction and Blue-Pippin size selection for large fragment whole genome libraries. Please inquire regarding iso-seq, amplicon or other types of libraries. If doing 10ug of DNA, costs are NOT double, enquire.
Fragment Analysis (AATI Femto Pulse and Fragment Analyzer) $60 per 11 samples (DNA or RNA).
Sanger Sequencing (ABI 3730xl) Samples can be submitted for immediate electrophoresis (charged for "Lane"), or BigDye Rxn, Clean-up, then electrophoresis (charged for "sequencing"). ByeDye v3.1 is also available for purchase at a rate of $410.00 per tube, which is typically enough for 1,600 reactions if used sparingly.
|Charge per sample||Internal (BYU PIs only)||External (costs go down with higher volume, see below)|
When will you get your ABI3730xl data?
If we have your samples before 5:00 PM, you will usually have your data the following morning, you may get it earlier depending on where your submission is in relation to other submissions. This same rule applies to Friday evening and Saturday morning. If you submit samples on Saturday, you will likely have your data Monday morning. If your samples are placed in our refrigerator after 5:00 PM, you may still get your data by the following morning.
Users submitting samples for Cycle Sequencing are requested to have your samples in our refrigerator by noon of Tuesdays and Thursdays, you will usually have your data by the following morning.
ABI Billing Rates
For internal BYU investigators your billing rate is listed at the top of the page. For External users you will need to pre-purchase your sequencing plans using the schedule listed below.
Sequencing Plans include BigDye reaction, clean-up and electrophoresis. Purified DNA templates and primers need to be provided at the right concentration.
Lane Plans include only capillary electrophoresis. Users need to perform BigDye reaction and clean-up reactions at their own cost. If you haven't already dried the samples down before submission, we will do so here prior to running them.
Internal - Sequencing Plans
External - Sequencing Plans
Internal - Lane Plans
External - Lane Plans
We have established a standing order purchase policy for external users in order to provide a more economical option for high volume external users.
Additional Explanations and Policies
The internal rates are for those PIs who are directly associated with BYU as faculty, staff, or student. All others are considered external users.
Lane charges refer to samples cycle sequenced and dried by the user before submission to the DNASC; Sequencing charges apply to plasmids or PCR products sent to be cycle sequenced by the facility.
We run positive controls with every sequencing and fragment analysis run. If we are unable to obtain quality sequence from our control, as well as from your DNA, you will not be assessed a charge or we will rerun your samples. We want you to be satisfied with the data we provide to you.
Billing is monthly and is based on the charges incurred from the 26th of the previous month to the 25th of the current month.
For more details or other questions please contact Dr. Edward Wilcox, Manager, DNASC. Phone:(801) 422-3647
- Dried samples, already cycle sequenced, ready for electrophoresis
- Individual samples (up to 31) must be in 1.7 ml microfuge tubes. Clearly label each tube numerically (1, 2, 3, etc) or alphanumerically (J-1, J-2, etc) on the lid. Long names written on the tubes create confusion and are not acceptable.
- Batches of 32 samples or more must be submitted in 96-well V bottom microplates or PCR plates, dried and covered with adhesive plate seal.
- Samples on 96-well plates will be loaded by column following the order: A1, B1 to H1, A2, B2 to H2 and so forth until H12. Your samples need to be submitted in this same order on your submitted plate. Follow the same format for partially filled plates and cross off any empty wells.
Fragment Analysis Samples
- We run Fragment Analysis/Gene Scan samples in batches of 16 up to 96 lanes.
- Samples need to be in 96-well PCR plates.
- Samples on 96-well plates will be loaded by column following the order: A1, B1 to H1, A2, B2 so forth until H12. Your samples need to be in that order on your plate. Follow the same format for partially filled plates and cross off any empty wells.
Purified PCR and plasmids submitted for cycle sequencing by the DNASC
- Samples need to be in 1.7 ml microfuge tubes, 96-well V microplates or PCR plates (32 samples or more).
- We need a volume of at least 4 µl.
- Primer must already be added to the sample (about 1.1 pmoles per µl, one primer per tube). The facility does not provide primers to users.
- Again, clearly label the tubes numerically or alphanumerically on the lid.
Sample brought in for post-PCR cleaning prior to cycle sequencing
- We currently use Exonuclease I/Antarctic Phosphatase to process these samples
- At least 10 µl of PCR reaction needs to be submitted for processing.
- Primers need to be aliquoted into individual tubes at a 3.2 µM concentration. We need 2 µl of primer per sample.
- The tray with your samples needs to have your name and submission code.
- Your cycle sequencing samples will be kept for a month from the arrival date.
- Electrophoresed samples will be stored @ -20 °C for 20 days. Reruns need to be requested ASAP.
- Zip files will be available on the DNASC server for download for 45 days. After 45 days, all data is stored on DVD. Users are responsible for backing up their own sequence data.
- All users are required to submit sample information on line. Samples without an on line submission cannot be processed.
DNASC Work Schedule
- Samples will be electrophoresed according to submission order but very large submissions (more than five 96 well plates) will be processed gradually in order to accommodate the needs and wants of smaller users.
- The DNASC is closed Saturdays, Sundays and major holidays.
Please contact the DNASC if you have any questions about our policies.