The Genomic and Bioinformatic Center (GBC) at Brigham Young University was established to help researchers and students process DNA samples efficiently and economically. By centralizing equipment and expertise, we have dramatically reduced the overall expense to the University and to individual labs needing DNA sequence, while increasing the efficiency and quality of the data generated. The GBC is committed to serving the BYU research community by providing advanced and efficient services for DNA sequence and DNA fragment acquisition and analysis.
To request services, BYU staff and students can login or create an account. External users can email dnasc@byu.edu.
Services
Below are some of the services provided by the GBC. Please see policies and pricing for more details.
Genomic Services
Custom PacBio HiFi sequencing on Revio using SPRQ chemistry, including DNA extraction, library prep and sequencing:
WGS
Iso-Seq
Kinnex
single cell Kinnex
Amplicon sequencing (amplicons <3Kbp)
Fiber Seq (Through collaboration)
Custom DNA Sequencing on 3730xl with dideoxy sequencing chemistry
DNA/RNA Fragment Analysis
Femto Pulse
Fragment Analyzer
Single Cell RNASeq (coming up)
Bioinformatics Services (coming up soon)
Pacbio Revio
HiFi sequencing alignments (genome reference needs to be provided)
Isoseq and single cell Isoseq analysis
Variant Calling
methylong: Extract methylation calls from long reads
RNA-Seq
rnaseq: Pipeline using STAR, HISAT2, RSEM, or Salmon with gene/isoform counts and quality control.
atacseq: ATAC-seq peak-calling and QC analysis pipeline
ChIP-Seq
chipseq: ChIP-seq peak-calling, QC and differential analysis pipeline.
DNA Methylation
methylseq: Methylation (Bisulfite-Sequencing) analysis pipeline using Bismark or bwa-meth + MethylDackel
Single Cell RNAseq:
scrnaseq: Single-cell RNA-Seq pipeline for barcode-based protocols such as 10x, DropSeq or SmartSeq, offering a variety of aligners and empty-droplet detection
scdownstream: A single cell transcriptomics pipeline for QC, integration and making the data presentable.
How to find us
The GBC is located in 4046 LSB. Our operating hours are 8:30am - 5:00pm Monday - Friday (except for BYU holidays)
The GBC is supported by Brigham Young University through the Department of Biology under the direction of Tommy W. Terooatea and managed by Emily Andersen.