Sampling Site Protocols

Saliva

Before Sampling

• Do not eat, drink, smoke or chew gum for 30 minutes before giving your saliva sample.
• Do not remove the plastic from the funnel lid

Sampling Procedure (2-5 minutes)

• Spit into the funnel until the amount of liquid saliva (not bubbles) reaches the fill line
• Hold the tube upright with one hand. Close the funnel lid with the other hand by firmly pushing the lid until you hear a loud click. The liquid in the lid will be released into the tube to mix with the saliva. Make sure the lid is closed tightly.
• Hold the tube upright. Unscrew the funnel from the tube
• Use the small cap to close the tube tightly
• Shake the capped tube for 5 seconds. Discard or recycle the funnel.

Blood

Venipuncture Protocol

•  Check subject armband and have subject verbally identify themselves with their initials.
•  Get blood draw supplies prepared: tourniquet, gloves, pre-labeled collection tubes, vacutainer holder, vacutainer needle, alcohol swab, cotton ball or gauze, band aid or tape.
•  Subject should be in a comfortable sitting or supine position.
•  Apply tourniquet. Select vein, insert needle into vein.
•  Insert collection tube into vacutainer holder and collect blood sample. If more than one sample is to be collected, use correct order of draw.
•  Once draw is completed, release tourniquet and withdraw needle while cotton/gauze is over venipuncture site. Once needle is completely out of subject’s arm, apply pressure to site with cotton ball or gauze.
•  Discard needle into sharps container.
•  Label blood collection tubes per protocol.
•  Check injection site for excessive bleeding. If bleeding has stopped, a band aid or cotton ball/gauze with coban may be applied.
•  Transport sample(s) to lab for processing.